METHOD OF ANALYSIS FOR DETERMINATION OF THE CHROMIUM (Cr) SPECIES IN WATER SAMPLES BY SPECTROPHOTOMETRY WITH DIPHENYLCARBAZIDE

Adam Wiryawan, Rurini Retnowati, Perry Burhan, Syekhfani Syekhfani

Abstract


Speciation of Chromium (Cr) is very important because of the toxicity of these metals depending on the oxidation number and its concentration is very low in the water system. Chromium occurs in the environment primarily in two valence states, trivalent Cr(III) and hexavalent Cr(VI). Chromium (III) is an essential micro-nutrients for the human body, while the Cr(VI) is highly toxic and carcino-gennic. Chromium(VI) in the water can be analyzed by spectrophotometry with diphenylcarbazide as reagent on pH=1 at maximum wavelength of 540 nm. The experimental result show that Cr(VI) can be analyzed using diphenylcarbazide in the concentration of 0.0015% and H3PO4 solution as acidic in 0.03 mol/L. The absorbance was measured at minutes 5 after preparation. There is interference from ion Fe(III) at least 6.0 ppm and this interference can be overcome by using 0.3 % NaF solution. The limit of detection of this method is -samples, mix of Cr(III) and Cr(VI), can be analyzed by using this method without oxidation by KMnO4 solution. While Cr(III)  in the artificial samples, mix of Cr(III) and Cr(VI), can be analyzed by using this method via oxidation by KMnO4 solution in the acidic media (H2SO4), the result of this analysis is the total concentration of Chromium. The concen-tration of Cr(III) can be calculate via subtract the total of Cr concentration by concentration of Cr(VI).

Keywords: speciation, Cr(III), Cr(VI), spectropho-tometry, diphenylcarbazide, Fe(III), interference.

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References


Lan, Y., Deng, B., Kim, C., Thornton, E.C., and Xu, H. 2005. Catalysis of Elemental Sulfur Nanoparticles on Chromium(VI) Reduction by Sulfide under Anaerobic Conditions. Environ. Sci. Technol.: 39, 2087-2094.

Capeans, P.P., Alonso, M.C.B., Barrera, A.B., Barrera, P.B. 2005. Chromium available fraction in aurosa sediments using a modified microve BCR protocol based on microwave assisted extraction. Talanta : 65(3), 678-685

Motomizu, S., Jitmanee, K., Oshima, M. 2003. Online collection/ concentration of trace metals spectroscopy detection via use of small-sized thin solid phase (STSP) column resin reactors. Application to speciation of Cr(III) and Cr(VI). Anal. Chim. Acta : 499, 149-155.

Gomez, V and Callao, M.P. 2006. Chromium determination and speciation since 2000. Trends in Anal.Chem., Vol. 25, No. 10, 1006-1015.

Emsley, J. 2001. Chromium. Nature's Building Blocks: An A-Z Guide to the Elements. Oxford University Press. Oxford. p. 495-498. ISBN 0198503407.

Kotaś, J. and Stasicka, Z. 2000. Chromium occurrence in the environment and methods of its speciation. Environmental Pollution : 107(3), 263 –283.

Cornelis, R. H., Caruso, .J. and Heumann, K.G. (eds.). 2005. Handbook ofElemental Speciation II: Species in the Environment, Food, Medicine & Occupational Health. John Wiley & Sons, Ltd. pp. 120-124.

Rakhunde, R., Deshpande, L. and Juneja, H.D. 2012. Chemical Speciation of Chromium in Water: A Review. Critical Rev. in Environ. Sci. and Tech.: 42, 776–810.

Anonymous. 1990. Peraturan Pemerintah R.I. No. 20 Tahun 1990 Tentang : Pengendalian Pencemaran Air.

Papassiopi, N., Kontoyianni, A., Vaxevanidou, K., Xenidis, A. 2009. Assessment of chromium biostabilization in contaminated soils using standard leaching and sequential extraction techniques. Science of the Total Environ.: 407, 925–936.

Harrington, C.F.; Clough, R.; Hansen, H.R.; Hill, S.J.; Pergantis, S.A. 2009. Atomic Spectrometry Update. Elemental speciation. J. Anal. Atomic Spectrom.: 24, 999-1025.

Nam, Sang-Ho and Kim, Yu-Na. 2012. An Investigation on the Extraction and Quantitation of a Hexavalent Chromium in Acrylonitrile Butadiene Styrene Copolymer (ABS) and Printed Circuit Board (PCB) by Ion Chromatography Coupled with ICP-AES. Bull. Korean Chem. Soc.: 33(6), 1967-1971.

Ku, J.C. and Eide, M. 2006. Hexavalent Chromium. Methods Development Team, Industrial Hygiene Chemistry Division, OSHA Salt Lake Technical Center, Sandy UT 84070-6406

Andruch, V., Telepcakov´a, M., Balogh, I.S., and Urbanov´a, N. 2003. Investigation of 2-[2-(4-methoxy-phenylamino)-vinyl]-1,3,3-trimethyl-3H-indolium chloride as a new reagent for the determination of chromium(VI). Microchim. Acta : 142, 109–113.

Pressman, M.A.S., and Aldstadt, J.H. 2003. A comparative study of diffusion samplers for the determination of hexavalent chromium by sequential injection spectrophotometry. Microchem. J. : 74, 47–57

Scindia, Y.M., Pandey, A.K., Reddy, A.V.R., and Manohar, S.B. 2002. Selective preconcentration and determination of chromium(VI) using a flat sheet polymer inclusion sorbent: Potential application for Cr(VI) determination in real samples. Anal. Chem.: 74, 4204–4212.

Scindia, Y.M., Pandey, A.K., Reddy, A.V.R., and Manohar, S.B. 2004. Chemically selective membrane optode for Cr(VI) determination in aqueous samples. Anal. Chim. Acta : 515, 311–321.

Ashley, K., Howe, A.M., Demangec, M. and Nygren, O. 2003. Sampling and analysis considerations for the determination of hexavalent chromium in workplace air. J. Environ. Monit.: 5, 707–716

Miller, J.N. and J. C. Miller. 2010. Statistics and Chemometrics for Analytical Chemistry. Sixth Ed. Ellis Horwood. Harlow. pp.124-127




DOI: http://dx.doi.org/10.21776/ub.jeest.2018.005.01.6

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