UV VIS STUDY IN INTERACTION ASTAXANTHIN AND GLYCATED BSA

Muhammad Irsyad Nur

Abstract


Astaxanthin antioxidant activity  has been reported to be more than 100 times greater than that of vitamin E against lipid peroxidation and approximately 550 times more potent than that of vitamin E for singlet oxygen quenching. The purpose of this study is to find out the best concentration of astaxanthin to reduce the uv absorbance from BSA glycated by using UV spectroscopy. Astaxanthin was dissolved in methanol at concentration (1 x 10-9– 1x10-10 mol/L) different concentrations, then added to BSA glycated 100mM and 500mM then observed with UV spectroscopy. From the UV spectroscopy observations at 284 nm wavelengths, the best results for astaxanthin concentration is 0.2x 10-9 and 1x10-10 mol/L , it shows the best concentration  on its effect to reduce the absorbance on BSA conformation

Keywords


Astaxanthin, BSA Glycated, Spectrum UV

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References


Ambigaipalan, P. & Shahidi, F. 2017. Bioactive peptides from shrimp shell processing discards: Antioxidant and biological activities. Journal of Functional Foods, 34: 7–17.

Bern, M., Sand, K.M.K., Nilsen, J., Sandlie, I. & Andersen, J.T. 2015. The role of albumin receptors in regulation of albumin homeostasis: Implications for drug delivery. Journal of Controlled Release, 211: 144–162.

Chen, W.P., Xiong, Y., Shi, Y.X., Hu, P.F., Bao, J.P. & Wu, L.D. 2014. Astaxanthin reduces matrix metalloproteinase expression in human chondrocytes. International Immunopharmacology, 19(1): 174–177.

Coral-Hinostroza, G.N. & Bjerkeng, B. 2002. Astaxanthin from the red crab langostilla (Pleuroncodes planipes): Optical R/S isomers and fatty acid moieties of astaxanthin esters. Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, 133(3): 437–444.

Fang, Y.-Z., Yang, S. & Wu, G. 2002. Regulation Of Physiological Systems By Nutrients Free Radicals, Antioxidants, and Nutrition. 9007(02): 872–879.

Fasano, M., Curry, S., Terreno, E., Galliano, M., Fanali, G., Narciso, P., Notari, S. & Ascenzi, P. 2005. Critical Review The Extraordinary Ligand Binding Properties of Human Serum Albumin. 57(December): 787–796.

GLAZER, A.N. & ROSENHECK, K. 1962. Solvent and conformational effects on the ultraviolet spectra of polypeptides and substituted amides. The Journal of biological chemistry, 237(12): 3674–3678.

Guerin-Dubourg, A., Catan, A., Bourdon, E. & Rondeau, P. 2012. Structural modifications of human albumin in diabetes. Diabetes and Metabolism, 38(2): 171–178.

He, Y., Yu, Z., He, J., Zhang, H., Liu, Y. & Lei, B. 2018. Ratiometric and selective fluorescent sensor for Fe(III) and bovine serum albumin based on energy transfer. Sensors and Actuators, B: Chemical, 262(Iii): 228–235.

Kawasaki, S., Mizuguchi, K., Sato, M., Kono, T. & Shimizu, H. 2013. A Novel Astaxanthin-Binding Photooxidative Stress-Inducible Aqueous Carotenoprotein from a Eukaryotic Microalga Isolated from Asphalt in Midsummer. 54(7): 1027–1040.

Kumirska, J., Kaczy, Z. & Bychowska, A. 2010. Application of Spectroscopic Methods for Structural Analysis of Chitin and Chitosan. 1567–1636.

Lange, R., Frank, J., Saldana, J.L. & Balny, C. 1996. Fourth derivative UV-spectroscopy of proteins under high pressure: I. Factors affecting the fourth derivative spectrum of the aromatic amino acids. European Biophysics Journal, 24(5): 277–283.

Li, X., Wang, G., Chen, D. & Lu, Y. 2015. β-Carotene and astaxanthin with human and bovine serum albumins. Food Chemistry, 179: 213–221.

Masuelli, M.A. 2013. Study of Bovine Serum Albumin Solubility in Aqueous Solutions by Intrinsic Viscosity Measurements. 2013.

Matthews, S.J., Ross, N.W., Lall, S.P. & Gill, T.A. 2006. Astaxanthin binding protein in Atlantic salmon. Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, 144(2): 206–214.

Ott, C., Jacobs, K., Haucke, E., Navarrete Santos, A., Grune, T. & Simm, A. 2014. Role of advanced glycation end products in cellular signaling. Redox Biology, 2(1): 411–429.

Rajendran, P., Nandakumar, N., Rengarajan, T., Palaniswami, R., Gnanadhas, E.N., Lakshminarasaiah, U., Gopas, J. & Nishigaki, I. 2014. Antioxidants and human diseases. Clinica Chimica Acta, 436: 332–347.

Roche, M., Rondeau, P., Singh, N.R., Tarnus, E. & Bourdon, E. 2008. The antioxidant properties of serum albumin. FEBS Letters, 582(13): 1783–1787.

Sadowska-Bartosz, I., Stefaniuk, I., Galiniak, S. & Bartosz, G. 2015. Glycation of bovine serum albumin by ascorbate in vitro: Possible contribution of the ascorbyl radical? Redox Biology, 6: 93–99.

Sakata, N., Moh, A. & Takebayashi, S. 2002. Contribution of superoxide to reduced antioxidant activity of glycoxidative serum albumin. Heart and Vessels, 17(1): 22–29.

Sowmya, P.R.R., Arathi, B.P., Vijay, K., Baskaran, V. & Lakshminarayana, R. 2017. Astaxanthin from shrimp efficiently modulates oxidative stress and allied cell death progression in MCF-7 cells treated synergistically with β-carotene and lutein from greens. Food and Chemical Toxicology, 106: 58–69.

Sowmya, R. & Sachindra, N.M. 2012. Evaluation of antioxidant activity of carotenoid extract from shrimp processing byproducts by in vitro assays and in membrane model system. Food Chemistry, 134(1): 308–314.

Taghavi, F., Habibi-Rezaei, M., Amani, M., Saboury, A.A. & Moosavi-Movahedi, A.A. 2017. The status of glycation in protein aggregation. International Journal of Biological Macromolecules,100:67–74.

Treatment, E.F., Jian, W., Wang, L., Wu, L. & Sun, Y. 2018. Physicochemical Properties of Bovine Serum Albumin-Glucose and Bovine Serum Albumin-Mannose Conjugates Prepared by Pulsed.

Ulrich, P., Ulrich, P. & Cerami, A. 2001. Protein Glycation, Diabetes, and Aging.

Wol, S.P., Jiang, Z.Y. & Huwr, J. V 1991. Diabetes Mellitus And Ageing. 10: 339–352.

Yamagishi, S.I. 2013. Advanced Glycation End-Products. Brenner’s Encyclopedia of Genetics: Second Edition, Elsevier Inc.

Yuan, C., Jin, Z. & Xu, X. 2012. Inclusion complex of astaxanthin with hydroxypropyl-β-cyclodextrin: UV, FTIR, 1H NMR and molecular modeling studies. Carbohydrate Polymers, 89(2): 492–496.

Zhu, T. & Row, K.H. 2013. Extraction of Astaxanthin from Shrimp Waste using Response Surface Methodology and a New Hybrid Organic-Inorganic Monolith. Separation Science and Technology (Philadelphia), 48(10): 1510–1517.




DOI: http://dx.doi.org/10.21776/ub.jeest.2019.006.01.4

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