Warsito Warsito, Noorhamdani Noorhamdani, Sukardi Sukardi, Suratmo Suratmo


Kaffir lime oil (C. hystrix DC.) is consist of a complex mixture of hydrocarbons terpenoids and terpenoids which contain oxygen are distributed in every part of the plant. In this research tested the antioxidant and antimicrobial activity, both against kaffir lime oil from twigs, leaves and rind including the main components citronellal. The antioxidant was evaluated by testing its ability to catch DPPH radical. Antimicrobial activity is measured by its ability to inhibit the growth of gram-negative bacteria, Escheria coli. Gas chromatography-mass spectrometry (GC-MS) is used to analyze chemical composition of kaffir lime oil. Value of IC50 of kaffir lime oil obtained was 6,43 (mL/mL); 6,83 (mL/mL); 9,48 (mL/mL) and 10,1 (mL/mL), consecutive to kaffir lime oil from rind, leaves, twig-leaf mixture and citronellal. Compared to the other kaffir lime oil and major components citronellal, kaffir lime oil from rind show the most vigorous activity in inhibiting growth of Escheria coli bacteria, by MIC values of 12,5 (mL/mL). GC-MS analysis showed that citronellal and citronellol as major component on kaffir lime oil from leaves and twig-leaf mixture, while the major component of kaffir lime oil from rind is b-pinene, limonene, citronellal dan terpinen-4-ol.


Keywords: kaffir lime oil, citronellal, citronellol, antioxidant, antimicrobial

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CARVALHO-FREITAS, M.I., COSTA, M., 2002, Anxiolytic andsedative effects of extracts and essential oil from Citrus aurantiumL. Biol. Pharm. Bull, 25: 1629-1633

CHOI, H.S., 2010, Functional Properties. Sawamura, M. (Ed.), Citrusessential oils – Flavour and Fragrance, pp. 132–145. Hoboken, NJ: John Wiley & SonsDOORANT, S.H, ROSE, L.C, SUHAIMI, H., MOHAMAD, H, ROZAINI, M.Z.H., TAI M., 2011, Preliminary evaluation on the antibacterial activities of Citrus hystrix oil emulsions stabilized by tween 80 and span 80, Int J Pharm Pharm Sci 3 (Suppl 2), 209–211.

ESTER R. CHAMORRO, SILVIA N. ZAMBÓN, WALTER G. MORALES, ALFREDO F. SEQUEIRA AND GUSTAVO A. VELASCO , 2012, Study of the Chemical Composition of Essential Oils by Gas Chromatography, National Technological University, Regional Faculty Resistance, UTN QUIMOBI Group, Argentina, www.intechopen.com

FAH CHUEAHONGTHONG, AMPASA VATE, A., OKONOGI, S., TIMA, S. AND S. ANUCHAPREEDA, 2011, Cytotoxic effects of crude kaffir lime (Citrus hystrix, DC.) leaf fractional extracts on leukemic cell lines, J. Med. Plants Res., Vol. 5,14, 3097-3105

HUNG, P. V., CHI, P. T. L, AND LAN-PHI, N. T., 2013, Comparison of antifungal activities of Vietnamese citrus essential oils. Natural Product Research 27: 506-508.

JONG SEOK B., KIM, S.S., LEE, J.A., OH, T.H., KIM, J.Y., LEE, N.O., HYUN, C.G., 2008, Chemical composition and biological activities of essential oils extracted from Korean endemic Citrus species. J. Microbiol. Biotechnol. 18: 74-79

LAN-PHI, N. T., HUNG, P. V., LAN-CHI, N. T. AND TUAN, P. D., 2015, Impact of Growing Locations and Genotypes on Antioxidant and Antimicrobial Activities of CitrusEssential Oils in Vietnam. Journal of Essential Oil Bearing Plants (published online).Doi:10.1080/0972060X.2015.1004124

LAN-PHI, N. T. AND VY, T. T., 2015, Chemical composition, antioxidant and antibacterial activities of peels’ essential oils of different pomelo varieties in the south of Vietnam, Inter. Food Res. Journal 22(6): 2426-2431

MAJNOONI, M.B., MANSOURI,K., GHOL-IVAND, M.B., MOSTAFAIE, A.,MOTLAGH, .R.M, AFNANZADE, N.S., ABOLGHASEMI,MM. AND PIRIYAEI, M., 2012, Chemical composition, cytotoxicity and antioxidant activities of the essential oil from the leaves of Citrus aurantium L. , African J. Biotech., Vol. 11(2), pp. 498-503.

MORAES, T.M., KUSHIMA, H., MOLEIRO, F.C., SANTOS, R.C., ROCHA, L.R, MARQUES, M.O., VILEGAS, W., HIRUMA-LIMA, C.A., 2009, Effects of limonene and essential oil from Citrus aurantium on gastric mucosa: Role of prostaglandins and gastric mucus secretion. Chem. Bio. Intera., 180: 499–505.

PULTRINI ADE, M., GALINDO, L.A., COSTA, M., 2006, Effect of the essential oil from Citrus aurantium L. in experimental anxiety models in mice. Life Sci. 78: 1720-1725

RUSLI, M. S., 2012, Effort and challenges for sustainable essential oil production in Indonesia, Conference Proceeding the IFEAT International Conference in Singapore, 161-169

SANGUINETTI, M.B., POSTERARO, L., ROMANO, F., BATTAGLIA, T., LOPIZZO, E., CAROLIS, D., FADDA, G., 2007, In vitroactivity of Citrus bergamia (bergamot) oil against clinical isolates of dermatophytes, J. Antimicrob. Chemother., 59: 305-308

SOUSA, OV., DEL-VECHIO-VIEIRA, G., ALVES, M.S., AÍLSON A., ARAÚJO, L., PINTO, M.A.O., AMARAL, M.P.H., RODARTE, M.P. AND M.A. C. KAPLAN, 2012, Chemica composition and biological activities of the essential oils from Duguetia lanceolata St. Hil. Barks, Molecules, 17, 11056-11066.

VIUDA-MARTOS, M., RUIZ-NAVAJAS, Y., FERNANDEZ - LOPEZ, J. AND PEREZ - ALVAREZ, J. ,2008, Antifungal activity of lemon (Citrus lemonL.), mandarin (Citrus reticulata L.), grapefruit (Citrus paradisi L.) and orange (Citrus sinensisL.) essential oils., Food Control, 19: 1130-1138.

WARSITO, UMI KALSUM, EDI PRIYO UTOMO, WAODE CAKRA NIRW ANA, 2013, Preparasi komponen-komponen minyak jeruk purut ( Citrus hystrix D.C.) sebagai bahan obat antimikroba dan antioksidan, Laporan Akhir Penelitian Unggulan Perguruan Tinggi, Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya.

YUNUS, M.M, 2009, Comparison of % composition of essential oil from peel and leaves of kaffir lime (Citrus hystrix) using hidrodistillation and solvent (ethyl acetate) extraction, Faculty of Applied Science, Universiti Teknologi MARA, Selangor

DOI: http://dx.doi.org/10.21776/ub.jeest.2017.004.01.3


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