ISOLATION AND PROFILLING OVIDUCT SPECIFICT GLYCOPROTEIN IN OVIDUCTAL FLUID OF KACANG GOAT (Capra aegagrus hircus)

Herlina Pratiwi

Abstract


Frozen semen supplemented by Oviduct Specific Glycoprotein (OVGP) is a new way to solve the decrease of in vitro fertility on goats caused by polyspermy and to increase the efficiency of artificial insemination on goats. Oviduct Specific Glycoprotein has associated with zona pellucida and localized in perivitelline space. The existence of Oviduct Specific Glycoprotein in perivitelline space may be linked to a mechanism that blocks polyspermic through vitelin block. Oviduct Specific Glycoprotein can be found in the fluid that was secreted by the oviduct (oviductal fluid). This study was aimed to determine Oviduct Specific Glycoprotein profile in the oviductal fluid of Kacang goats Malang district, West Java Province, Indonesia. Oviduct Specific Glycoproteins were collected from an oviductal liquid of kacang goats and samples were loaded to 12% SDS-PAGE to determine the weight of the molecule of Oviduct Specific Glycoprotein, further confirmed by western blot. The result of this study showed that molecule weight of Oviduct Specific Glycoprotein been collected from oviductal liquid was approximately 55-65 kDa. After being confirmed by western blotting the molecule weight of Oviduct Specific Glycoprotein was 65 kDa.


Full Text:

PDF

References


Hunter RH. 2003. Reflections upon sperm–endosalpingeal and sperm–zona pellucida interactions in vivo and in vitro. Reproduction in Domestic Animals;38 147–154.

McCauley, T.C., W.C. Buhi, G.M. Wu, J. Mao,J.N Caamano, B.A. Didion, and B.N. Day. 2004. Oviduct-Specific Glycoprotein Modulates Sperm-Zona Binding and Improves Efficiency of Porcine Fertilization In vitro. Biology of Reproduction 69: 828–834.M. P. Brown and K. Austin, Appl. Phys. Letters 85, 2503–2504.

Coy P., and M. Aviles. 2010. What Controls Polyspermy in Mammals, the Oviduct or the Oocyte?. Biological Reviews;85: 593–605.

Buhi, W.C. 2002. Characterization and Biological Roles of Oviduct-specific, Oestrogen-dependent Glycoprotein. Reproduction;123: 355-362.

Bergeron A, Vandenberg G, Proulx D, Bailey JL. 2002. Comparison of extenders, dilution ratios and theophylline addition on the function of cryopreserved walleye semen. Theriogenology; 57(3):1061-71.

Moura, A.A.,H. Koc, D.A. Chapman and G.J. Killian. 2006. Identification of accessory sex gland fluid proteins as related to fertility indexes of dairy bulls: a proteomic approach. J. Androl; 27: 201–211.

Goncalves, R. F., D. A. Chapman, R. P. Bertolla, I. Eder and G. J. Killian. 2008. Pre-treatment of cattle semen or oocytes with purified milk osteopontin affects in vitro fertilization and embryo development (abstract). AnimReprod Sci.;108(3-4):375-83.

Van oss, CJ. 1989. Energetic of cell-cell and cell biopolymer interaction. Cell Biophys; 14: 1-16.

Aulanni’am. 2005. Protein dan Analisisnya. First Edition. Publisher: Citra Mentari Group. Malang. Pp: 19-27.

Lapointe, S., C. Legare, C. Gaudreault, R. Sullivan, and M. A. Sirard. 1998. cDNA sequence and deduced amino acid sequence of bovine oviductal fluid catalase. Mol. Reprod. Dev; 51:265–273.

Pradeep M.A., J. Jagadeesh, A.K. De, J.K. Kaushik, D. Malakar, S. Kumar, A.K. Dang, S.K. Das, and A.K. Mohanty. 2011. Purification, sequence characterization and effect of goat oviduct-specific glycoprotein on in vitro embryo development. Theriogenology ;75: 1005–1015.

Malette B, Bleau G. 1993. Biochemical characterization of hamster oviductin as a sulfated zona pellucida-binding glycoprotein. Biochem J; 295:437– 45




DOI: http://dx.doi.org/10.21776/ub.jeest.2017.004.01.7

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.